뼈흡수유도호르몬이 ROS17/2.8세포로부터 Nitric Oxide 형성에 미치는 영향
Effects of osteotropic hormones on the nitric oxide production in culture of ROS17/2.8 cells
소속 상세정보
고선일/Ko SY
김민성/한원정/김세원/김정근/Kim MS/Han WJ/Kim SW/Kim JK
KMID : 0896020050350030127
Abstract
Purpose : We performed the present study to investigate whether osteotropic hormomes play roles on the nitric oxide (NO) production in culture of ROS17/2.8 osteoblastic cells.
Materials and Methods : The osteoblastic cell line ROS17/2.8 cells were cultured in F12 medium supplemented with 5% fetal bovine serum (FBS) at 37�C in a humidified atmosphere of 5% CO2 in air. ROS17/2.8 cells were plated in 96-well plates at a density of 2-3?103 cells/well and grown to confluence. Then the cells were pretreated
with osteotropic hormones (parathyroid hormone (PTH) 20-500 ng/mL, 1, 25-dihydroxycholecalciferol (1, 25[OH]2D3) 1-100 nM; prostaglandin E2 (PGE2) 20-500 ng/mL) in the medium supplemented with 0.4% FBS for 72 hours and the cells were treated with cytokines (TNF꺙 and IFN꺛) in phenol red-free F12 medium for an additional
48 hours. NO synthesis was assessed by measuring the nitrite anion concentration, the reaction product of NO, in the cell culture medium using Griess reagent.
Results : PTH and 1, 25[OH]2D3 pretreatment induced a significant increase in NO production in the presence of TNF꺙 and IFN꺛. PGE2 slightly induced NO production compared to the control group. But, PGE2 pretreatment did not affect in NO production in the presence of TNF꺙 and IFN꺛.
Conclusions : These results suggest that the actions of osteotropic hormones in bone metabolism may be partially mediated by NO in the presence of cytokines. (Korean J Oral Maxillofac Radiol 2005; 35 : 127-31)
키워드
Nitric Oxide; Osteotropic Hormones; Cytokines; Osteoblasts
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